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We hypothesized that normobaric hyperoxia (NBOH) would inhibit the development of endotoxin-induced acute lung injury (ALI) in the rat. Male Sprague-Dawley rats were randomized to receive either vehicle or 10 mg/kg endotoxin (ET; Salmonella enteritidis 074:B6) in a 2 x 2 factorial design with either normoxia or NBOH. Normoxia and NBOH groups were also randomized to receive either saline or 10 mg/kg of hydrocortisone. Rats were subsequently ventilated for 4 h with either 21% O2/79% N2 or 95% O2/5% N2. End-tidal CO2 was maintained at 2-4% in all groups. After 4 h of mechanical ventilation, total cell counts in bronchoalveolar lavage (BAL) and wet-to-dry weight ratios of the lung were increased (p < 0.05) in the ET groups compared to controls. However, this effect was not seen in the ET + NBOH groups. The ET + NBOH + hydrocortisone groups had similar BAL cell counts to the ET + NBOH + saline groups. These data suggest that NBOH inhibits the development of lung injury caused by ET.
One of the things that I like about this plug-in is that it is very easy to use. You can download it from the author's website, http://www.imagenomic.com. I am not affiliated with them. I just use their plug-ins.
The author of the plug-in, David Kessens, always responds to my questions. If you have any questions, you can contact him. David Kessens, jkessens@imagenomic.com. If you have any questions, you can contact him. David Kessens, jkessens@imagenomic.com.
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